A human brain with ~86.1 billion neurons forms ~100 trillion connections whereas a simple multicellular organism such as the nematode, C. elegans with 302 neurons establishes ~7000 connections. Building this intricate neural network in an orderly and functional manner is essential to the development and function of an organism. The molecular mechanisms that create and maintain the neural circuits are poorly understood. Microtubules, alpha, and beta-tubulin polymers are critical structural components of neurons. Several microtubule-related proteins are linked to mutations in neurological and neurodevelopment disorders. Despite the central role of the microtubule in the neurons, little is known about the function of the microtubule cytoskeleton in sculpting the neural circuits. My research aims to gain a detailed mechanistic understanding of how the microtubule cytoskeleton builds and maintains the neuronal circuitry.